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S, we included the inter-individual variability, the variability within laboratories, the variability between laboratories and the remaining variability (variance components, that could be attributable to technicians and laboratory procedures) in our statistic model as described below. The remaining factors are considered as stable due to several reasons. The intraindividual variability is regarded as low in coagulation tests and the interval between the two measurements is very short (2? hours) [11]. Influence of the platform was addressed as calculations have been PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/13485127 done by every platform. The assay designs were identical by platform. Preanalytic factors were considered by applying a strict protocol and using the same technician for serial measurements. Storage and transport of the samples were identical for all laboratories. To avoid influences of different batches of reagents, all determinations were done in one batch in every laboratory.All nine haemostasiological laboratories of tertiary hospitals in Switzerland participated in this investigation (In alphabetical order; Cantonal Hospital Aarau, University Hospital of Basel, Inselspital University Hospital of Berne, University Hospital of Geneva, University Hospital of Lausanne, Regional Hospital of Locarno, Cantonal Hospital of Lucerne, Cantonal Hospital of St. Gallen, University Hospital of Zurich). One laboratory was excluded from analysis of between-laboratory variability because of a unique platform. The remaining eight laboratories used one out of three platforms. Details of coagulometers, assays and reagents used are given in Table 1. For determination of variability between laboratories, local protocols were used for testing samples. All laboratories used strict internal and external quality assessment measures, conducted formal test evaluation and are accredited to the Swiss Accreditation Service (SAS). Coagulation tests have been done in accordance with international guidelines [12]. Fibrinogen was determined in plasma samples of both points in time. Prothrombin time (PT), factors II, V, VII, VIII, IX, X, XI and XIII were determined only for the first sample to avoid possible interferences with the second investigation conducted [10].Statistical analysisTo study the variability between the different laboratories, Fmoc-Oic-OH we performed analyses of variance (ANOVAs) and used the variance components procedure. By platform, the variance components of (i) the subjects, (ii) the laboratory and the technician (including the measurement error of the platform) and (iii) the total variance were obtained for fibrinogen as well as (i) and (iii) for the remaining factors. Based on the variance components, we calculated the intraclass correlation coefficients (ICC) for all factors using the variance component of the subjects in the numerator and the total variance in the 3-Chloro-5-cyclopropylpyridine denominator. Thus, the variation of the measurements between laboratories can be demonstrated in PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/10811056 relation to theNagler et al. Thrombosis Journal 2013, 11:6 http://www.thrombosisjournal.com/content/11/1/Table 1 Participating laboratories with coagulometers, assays and reagents usedLaboratory?Platform A B C D E F G* H I Instrumentation Laboratory ACL Top 700 Siemens BCS-XP Roche STA-R Sysmex CA7000 Siemens BCS-XP Siemens BCS Sysmex CA1500 Siemens BCS-XP Roche STA-R Extrinsic coagulation factors Assay Clotting test (photometric) Clotting test (photometric) Clotting test (viscosity) Clotting test (photometric) Clotting test (photomet.